Review



human skmc line  (PromoCell)


Bioz Verified Symbol PromoCell is a verified supplier
Bioz Manufacturer Symbol PromoCell manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 94
      Buy from Supplier

    Structured Review

    PromoCell human skmc line
    Series of in vitro analysis using <t>human</t> <t>skeletal</t> muscle cells (SkMCs). (A) Overexpression of miR‐203 expression 48 h after transfection in <t>SkMC</t> line. (B) Effect of miR‐203 overexpression on SkMC proliferation as assessed by MTT assay. (C) Cell cycle analysis demonstrated that G 0 /G 1 fraction was increased after miR‐203 overexpression. (D, E) Apoptosis assay to investigate the population of apoptotic cells and viable cells of SkMCs after miR‐203 overexpression. Apoptosis rates were measured by annexin V and 7‐amino‐actinomycin D (7‐AAD) staining, and apoptotic cells were calculated as upper right and lower right (D): negative control (CTRL): right panel; miR‐203 mimic: left panel. Rate of apoptotic cells was significantly increased after miR‐203 overexpression ( P < 0.05, lower panel). The number of viable cancer cells was also calculated by MTT assay and showed that miR‐203 up‐regulation decreased the number of viable SkMCs (E). (F) Prediction of miR‐203 target gene via four different miRNA target prediction tools (miRMap, Microt4, Targetscan, and RNAhybrid). (G) Expression profile of apoptosis‐related genes about putative targets of miR‐203 showed that overexpression of miR‐203 suppressed BIRC5 expression in SkMCs. Expression profile of apoptosis‐related genes about putative targets of miR‐203 showed that overexpression of miR‐203 suppressed BIRC5 expression in SkMCs. (H) BIRC5 protein was expressed in both nucleus and cytoplasm in SkMCs and was down‐regulated in miR‐203 mimic‐transfected cells compared with negative CTRL cells. Each value represents the mean ± standard error. OD, optical density.
    Human Skmc Line, supplied by PromoCell, used in various techniques. Bioz Stars score: 94/100, based on 95 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+skmc+line/Human+Skeletal+Muscle+Cells/pmc06596405-130-1-7
    Average 94 stars, based on 95 article reviews
    human skmc line - by Bioz Stars, 2026-09
    94/100 stars

    Images

    1) Product Images from "Circulating miR‐203 derived from metastatic tissues promotes myopenia in colorectal cancer patients"

    Article Title: Circulating miR‐203 derived from metastatic tissues promotes myopenia in colorectal cancer patients

    Journal: Journal of Cachexia, Sarcopenia and Muscle

    doi: 10.1002/jcsm.12403

    Series of in vitro analysis using human skeletal muscle cells (SkMCs). (A) Overexpression of miR‐203 expression 48 h after transfection in SkMC line. (B) Effect of miR‐203 overexpression on SkMC proliferation as assessed by MTT assay. (C) Cell cycle analysis demonstrated that G 0 /G 1 fraction was increased after miR‐203 overexpression. (D, E) Apoptosis assay to investigate the population of apoptotic cells and viable cells of SkMCs after miR‐203 overexpression. Apoptosis rates were measured by annexin V and 7‐amino‐actinomycin D (7‐AAD) staining, and apoptotic cells were calculated as upper right and lower right (D): negative control (CTRL): right panel; miR‐203 mimic: left panel. Rate of apoptotic cells was significantly increased after miR‐203 overexpression ( P < 0.05, lower panel). The number of viable cancer cells was also calculated by MTT assay and showed that miR‐203 up‐regulation decreased the number of viable SkMCs (E). (F) Prediction of miR‐203 target gene via four different miRNA target prediction tools (miRMap, Microt4, Targetscan, and RNAhybrid). (G) Expression profile of apoptosis‐related genes about putative targets of miR‐203 showed that overexpression of miR‐203 suppressed BIRC5 expression in SkMCs. Expression profile of apoptosis‐related genes about putative targets of miR‐203 showed that overexpression of miR‐203 suppressed BIRC5 expression in SkMCs. (H) BIRC5 protein was expressed in both nucleus and cytoplasm in SkMCs and was down‐regulated in miR‐203 mimic‐transfected cells compared with negative CTRL cells. Each value represents the mean ± standard error. OD, optical density.
    Figure Legend Snippet: Series of in vitro analysis using human skeletal muscle cells (SkMCs). (A) Overexpression of miR‐203 expression 48 h after transfection in SkMC line. (B) Effect of miR‐203 overexpression on SkMC proliferation as assessed by MTT assay. (C) Cell cycle analysis demonstrated that G 0 /G 1 fraction was increased after miR‐203 overexpression. (D, E) Apoptosis assay to investigate the population of apoptotic cells and viable cells of SkMCs after miR‐203 overexpression. Apoptosis rates were measured by annexin V and 7‐amino‐actinomycin D (7‐AAD) staining, and apoptotic cells were calculated as upper right and lower right (D): negative control (CTRL): right panel; miR‐203 mimic: left panel. Rate of apoptotic cells was significantly increased after miR‐203 overexpression ( P < 0.05, lower panel). The number of viable cancer cells was also calculated by MTT assay and showed that miR‐203 up‐regulation decreased the number of viable SkMCs (E). (F) Prediction of miR‐203 target gene via four different miRNA target prediction tools (miRMap, Microt4, Targetscan, and RNAhybrid). (G) Expression profile of apoptosis‐related genes about putative targets of miR‐203 showed that overexpression of miR‐203 suppressed BIRC5 expression in SkMCs. Expression profile of apoptosis‐related genes about putative targets of miR‐203 showed that overexpression of miR‐203 suppressed BIRC5 expression in SkMCs. (H) BIRC5 protein was expressed in both nucleus and cytoplasm in SkMCs and was down‐regulated in miR‐203 mimic‐transfected cells compared with negative CTRL cells. Each value represents the mean ± standard error. OD, optical density.

    Techniques Used: In Vitro, Over Expression, Expressing, Transfection, MTT Assay, Cell Cycle Assay, Apoptosis Assay, Staining, Negative Control

    Related Articles

    In Vitro:

    Article Title: Circulating miR‐203 derived from metastatic tissues promotes myopenia in colorectal cancer patients
    Article Snippet: The human SkMC line was purchased from Promo Cell (Heidelberg, Germany) and maintained in Skeletal Muscle Cell Growth Medium (Promo Cell) at 37 °C in 5% humidified CO 2 atmosphere.

    Over Expression:

    Article Title: Circulating miR‐203 derived from metastatic tissues promotes myopenia in colorectal cancer patients
    Article Snippet: The human SkMC line was purchased from Promo Cell (Heidelberg, Germany) and maintained in Skeletal Muscle Cell Growth Medium (Promo Cell) at 37 °C in 5% humidified CO 2 atmosphere.

    Expressing:

    Article Title: Circulating miR‐203 derived from metastatic tissues promotes myopenia in colorectal cancer patients
    Article Snippet: The human SkMC line was purchased from Promo Cell (Heidelberg, Germany) and maintained in Skeletal Muscle Cell Growth Medium (Promo Cell) at 37 °C in 5% humidified CO 2 atmosphere.

    Transfection:

    Article Title: Circulating miR‐203 derived from metastatic tissues promotes myopenia in colorectal cancer patients
    Article Snippet: The human SkMC line was purchased from Promo Cell (Heidelberg, Germany) and maintained in Skeletal Muscle Cell Growth Medium (Promo Cell) at 37 °C in 5% humidified CO 2 atmosphere.

    MTT Assay:

    Article Title: Circulating miR‐203 derived from metastatic tissues promotes myopenia in colorectal cancer patients
    Article Snippet: The human SkMC line was purchased from Promo Cell (Heidelberg, Germany) and maintained in Skeletal Muscle Cell Growth Medium (Promo Cell) at 37 °C in 5% humidified CO 2 atmosphere.

    Cell Cycle Assay:

    Article Title: Circulating miR‐203 derived from metastatic tissues promotes myopenia in colorectal cancer patients
    Article Snippet: The human SkMC line was purchased from Promo Cell (Heidelberg, Germany) and maintained in Skeletal Muscle Cell Growth Medium (Promo Cell) at 37 °C in 5% humidified CO 2 atmosphere.

    Apoptosis Assay:

    Article Title: Circulating miR‐203 derived from metastatic tissues promotes myopenia in colorectal cancer patients
    Article Snippet: The human SkMC line was purchased from Promo Cell (Heidelberg, Germany) and maintained in Skeletal Muscle Cell Growth Medium (Promo Cell) at 37 °C in 5% humidified CO 2 atmosphere.

    Staining:

    Article Title: Circulating miR‐203 derived from metastatic tissues promotes myopenia in colorectal cancer patients
    Article Snippet: The human SkMC line was purchased from Promo Cell (Heidelberg, Germany) and maintained in Skeletal Muscle Cell Growth Medium (Promo Cell) at 37 °C in 5% humidified CO 2 atmosphere.

    Negative Control:

    Article Title: Circulating miR‐203 derived from metastatic tissues promotes myopenia in colorectal cancer patients
    Article Snippet: The human SkMC line was purchased from Promo Cell (Heidelberg, Germany) and maintained in Skeletal Muscle Cell Growth Medium (Promo Cell) at 37 °C in 5% humidified CO 2 atmosphere.



    Similar Products

    skmc human striated skeletal muscle cell line  (ATCC)
    95
    ATCC skmc human striated skeletal muscle cell line
    Skmc Human Striated Skeletal Muscle Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+skmc+line/Primary+Skeletal+Muscle+Cells/10__1007_slash_bf02639441-1606-3-22
    Average 95 stars, based on 1 article reviews
    skmc human striated skeletal muscle cell line - by Bioz Stars, 2026-09
    95/100 stars
    		  Buy from Supplier
    human skmc line  (PromoCell)
    94
    PromoCell human skmc line
    Series of in vitro analysis using <t>human</t> <t>skeletal</t> muscle cells (SkMCs). (A) Overexpression of miR‐203 expression 48 h after transfection in <t>SkMC</t> line. (B) Effect of miR‐203 overexpression on SkMC proliferation as assessed by MTT assay. (C) Cell cycle analysis demonstrated that G 0 /G 1 fraction was increased after miR‐203 overexpression. (D, E) Apoptosis assay to investigate the population of apoptotic cells and viable cells of SkMCs after miR‐203 overexpression. Apoptosis rates were measured by annexin V and 7‐amino‐actinomycin D (7‐AAD) staining, and apoptotic cells were calculated as upper right and lower right (D): negative control (CTRL): right panel; miR‐203 mimic: left panel. Rate of apoptotic cells was significantly increased after miR‐203 overexpression ( P < 0.05, lower panel). The number of viable cancer cells was also calculated by MTT assay and showed that miR‐203 up‐regulation decreased the number of viable SkMCs (E). (F) Prediction of miR‐203 target gene via four different miRNA target prediction tools (miRMap, Microt4, Targetscan, and RNAhybrid). (G) Expression profile of apoptosis‐related genes about putative targets of miR‐203 showed that overexpression of miR‐203 suppressed BIRC5 expression in SkMCs. Expression profile of apoptosis‐related genes about putative targets of miR‐203 showed that overexpression of miR‐203 suppressed BIRC5 expression in SkMCs. (H) BIRC5 protein was expressed in both nucleus and cytoplasm in SkMCs and was down‐regulated in miR‐203 mimic‐transfected cells compared with negative CTRL cells. Each value represents the mean ± standard error. OD, optical density.
    Human Skmc Line, supplied by PromoCell, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+skmc+line/Human+Skeletal+Muscle+Cells/pmc06596405-130-1-7
    Average 94 stars, based on 1 article reviews
    human skmc line - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier
    skmc cell line  (PromoCell)
    94
    PromoCell skmc cell line
    Series of in vitro analysis using <t>human</t> <t>skeletal</t> muscle cells (SkMCs). (A) Overexpression of miR‐203 expression 48 h after transfection in <t>SkMC</t> line. (B) Effect of miR‐203 overexpression on SkMC proliferation as assessed by MTT assay. (C) Cell cycle analysis demonstrated that G 0 /G 1 fraction was increased after miR‐203 overexpression. (D, E) Apoptosis assay to investigate the population of apoptotic cells and viable cells of SkMCs after miR‐203 overexpression. Apoptosis rates were measured by annexin V and 7‐amino‐actinomycin D (7‐AAD) staining, and apoptotic cells were calculated as upper right and lower right (D): negative control (CTRL): right panel; miR‐203 mimic: left panel. Rate of apoptotic cells was significantly increased after miR‐203 overexpression ( P < 0.05, lower panel). The number of viable cancer cells was also calculated by MTT assay and showed that miR‐203 up‐regulation decreased the number of viable SkMCs (E). (F) Prediction of miR‐203 target gene via four different miRNA target prediction tools (miRMap, Microt4, Targetscan, and RNAhybrid). (G) Expression profile of apoptosis‐related genes about putative targets of miR‐203 showed that overexpression of miR‐203 suppressed BIRC5 expression in SkMCs. Expression profile of apoptosis‐related genes about putative targets of miR‐203 showed that overexpression of miR‐203 suppressed BIRC5 expression in SkMCs. (H) BIRC5 protein was expressed in both nucleus and cytoplasm in SkMCs and was down‐regulated in miR‐203 mimic‐transfected cells compared with negative CTRL cells. Each value represents the mean ± standard error. OD, optical density.
    Skmc Cell Line, supplied by PromoCell, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+skmc+line/SkMC-c+Human+Skeletal+Muscle+Cells/bio_rxiv__2025__07__14__663986-216-0-11
    Average 94 stars, based on 1 article reviews
    skmc cell line - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier
    human pd myoblast cell line  (PromoCell)
    94
    PromoCell human pd myoblast cell line
    Series of in vitro analysis using <t>human</t> <t>skeletal</t> muscle cells (SkMCs). (A) Overexpression of miR‐203 expression 48 h after transfection in <t>SkMC</t> line. (B) Effect of miR‐203 overexpression on SkMC proliferation as assessed by MTT assay. (C) Cell cycle analysis demonstrated that G 0 /G 1 fraction was increased after miR‐203 overexpression. (D, E) Apoptosis assay to investigate the population of apoptotic cells and viable cells of SkMCs after miR‐203 overexpression. Apoptosis rates were measured by annexin V and 7‐amino‐actinomycin D (7‐AAD) staining, and apoptotic cells were calculated as upper right and lower right (D): negative control (CTRL): right panel; miR‐203 mimic: left panel. Rate of apoptotic cells was significantly increased after miR‐203 overexpression ( P < 0.05, lower panel). The number of viable cancer cells was also calculated by MTT assay and showed that miR‐203 up‐regulation decreased the number of viable SkMCs (E). (F) Prediction of miR‐203 target gene via four different miRNA target prediction tools (miRMap, Microt4, Targetscan, and RNAhybrid). (G) Expression profile of apoptosis‐related genes about putative targets of miR‐203 showed that overexpression of miR‐203 suppressed BIRC5 expression in SkMCs. Expression profile of apoptosis‐related genes about putative targets of miR‐203 showed that overexpression of miR‐203 suppressed BIRC5 expression in SkMCs. (H) BIRC5 protein was expressed in both nucleus and cytoplasm in SkMCs and was down‐regulated in miR‐203 mimic‐transfected cells compared with negative CTRL cells. Each value represents the mean ± standard error. OD, optical density.
    Human Pd Myoblast Cell Line, supplied by PromoCell, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+skmc+line/SkMC-c+Human+Skeletal+Muscle+Cells/10__36922_slash_gpd__1760-61-1-33
    Average 94 stars, based on 1 article reviews
    human pd myoblast cell line - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier
    human myoblasts cell line  (PromoCell)
    94
    PromoCell human myoblasts cell line
    Series of in vitro analysis using <t>human</t> <t>skeletal</t> muscle cells (SkMCs). (A) Overexpression of miR‐203 expression 48 h after transfection in <t>SkMC</t> line. (B) Effect of miR‐203 overexpression on SkMC proliferation as assessed by MTT assay. (C) Cell cycle analysis demonstrated that G 0 /G 1 fraction was increased after miR‐203 overexpression. (D, E) Apoptosis assay to investigate the population of apoptotic cells and viable cells of SkMCs after miR‐203 overexpression. Apoptosis rates were measured by annexin V and 7‐amino‐actinomycin D (7‐AAD) staining, and apoptotic cells were calculated as upper right and lower right (D): negative control (CTRL): right panel; miR‐203 mimic: left panel. Rate of apoptotic cells was significantly increased after miR‐203 overexpression ( P < 0.05, lower panel). The number of viable cancer cells was also calculated by MTT assay and showed that miR‐203 up‐regulation decreased the number of viable SkMCs (E). (F) Prediction of miR‐203 target gene via four different miRNA target prediction tools (miRMap, Microt4, Targetscan, and RNAhybrid). (G) Expression profile of apoptosis‐related genes about putative targets of miR‐203 showed that overexpression of miR‐203 suppressed BIRC5 expression in SkMCs. Expression profile of apoptosis‐related genes about putative targets of miR‐203 showed that overexpression of miR‐203 suppressed BIRC5 expression in SkMCs. (H) BIRC5 protein was expressed in both nucleus and cytoplasm in SkMCs and was down‐regulated in miR‐203 mimic‐transfected cells compared with negative CTRL cells. Each value represents the mean ± standard error. OD, optical density.
    Human Myoblasts Cell Line, supplied by PromoCell, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+skmc+line/SkMC-c+Human+Skeletal+Muscle+Cells/pm35406586-53-0-23
    Average 94 stars, based on 1 article reviews
    human myoblasts cell line - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier
    human dmd cell lines  (PromoCell)
    94
    PromoCell human dmd cell lines
    Series of in vitro analysis using <t>human</t> <t>skeletal</t> muscle cells (SkMCs). (A) Overexpression of miR‐203 expression 48 h after transfection in <t>SkMC</t> line. (B) Effect of miR‐203 overexpression on SkMC proliferation as assessed by MTT assay. (C) Cell cycle analysis demonstrated that G 0 /G 1 fraction was increased after miR‐203 overexpression. (D, E) Apoptosis assay to investigate the population of apoptotic cells and viable cells of SkMCs after miR‐203 overexpression. Apoptosis rates were measured by annexin V and 7‐amino‐actinomycin D (7‐AAD) staining, and apoptotic cells were calculated as upper right and lower right (D): negative control (CTRL): right panel; miR‐203 mimic: left panel. Rate of apoptotic cells was significantly increased after miR‐203 overexpression ( P < 0.05, lower panel). The number of viable cancer cells was also calculated by MTT assay and showed that miR‐203 up‐regulation decreased the number of viable SkMCs (E). (F) Prediction of miR‐203 target gene via four different miRNA target prediction tools (miRMap, Microt4, Targetscan, and RNAhybrid). (G) Expression profile of apoptosis‐related genes about putative targets of miR‐203 showed that overexpression of miR‐203 suppressed BIRC5 expression in SkMCs. Expression profile of apoptosis‐related genes about putative targets of miR‐203 showed that overexpression of miR‐203 suppressed BIRC5 expression in SkMCs. (H) BIRC5 protein was expressed in both nucleus and cytoplasm in SkMCs and was down‐regulated in miR‐203 mimic‐transfected cells compared with negative CTRL cells. Each value represents the mean ± standard error. OD, optical density.
    Human Dmd Cell Lines, supplied by PromoCell, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+skmc+line/SkMC-c+Human+Skeletal+Muscle+Cells/bio_rxiv__207480-31-0-12
    Average 94 stars, based on 1 article reviews
    human dmd cell lines - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier
    human skeletal muscle cell line skmc cc2561  (Cambrex)
    90
    Cambrex human skeletal muscle cell line skmc cc2561
    Series of in vitro analysis using <t>human</t> <t>skeletal</t> muscle cells (SkMCs). (A) Overexpression of miR‐203 expression 48 h after transfection in <t>SkMC</t> line. (B) Effect of miR‐203 overexpression on SkMC proliferation as assessed by MTT assay. (C) Cell cycle analysis demonstrated that G 0 /G 1 fraction was increased after miR‐203 overexpression. (D, E) Apoptosis assay to investigate the population of apoptotic cells and viable cells of SkMCs after miR‐203 overexpression. Apoptosis rates were measured by annexin V and 7‐amino‐actinomycin D (7‐AAD) staining, and apoptotic cells were calculated as upper right and lower right (D): negative control (CTRL): right panel; miR‐203 mimic: left panel. Rate of apoptotic cells was significantly increased after miR‐203 overexpression ( P < 0.05, lower panel). The number of viable cancer cells was also calculated by MTT assay and showed that miR‐203 up‐regulation decreased the number of viable SkMCs (E). (F) Prediction of miR‐203 target gene via four different miRNA target prediction tools (miRMap, Microt4, Targetscan, and RNAhybrid). (G) Expression profile of apoptosis‐related genes about putative targets of miR‐203 showed that overexpression of miR‐203 suppressed BIRC5 expression in SkMCs. Expression profile of apoptosis‐related genes about putative targets of miR‐203 showed that overexpression of miR‐203 suppressed BIRC5 expression in SkMCs. (H) BIRC5 protein was expressed in both nucleus and cytoplasm in SkMCs and was down‐regulated in miR‐203 mimic‐transfected cells compared with negative CTRL cells. Each value represents the mean ± standard error. OD, optical density.
    Human Skeletal Muscle Cell Line Skmc Cc2561, supplied by Cambrex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+skmc+line/skmc+cambrex++cc+2561/10__4172_slash_2157___7013__s4___001-94-17-22
    Average 90 stars, based on 1 article reviews
    human skeletal muscle cell line skmc cc2561 - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    human skeletal muscle cell line skmc  (Lonza)
    90
    Lonza human skeletal muscle cell line skmc
    Series of in vitro analysis using <t>human</t> <t>skeletal</t> muscle cells (SkMCs). (A) Overexpression of miR‐203 expression 48 h after transfection in <t>SkMC</t> line. (B) Effect of miR‐203 overexpression on SkMC proliferation as assessed by MTT assay. (C) Cell cycle analysis demonstrated that G 0 /G 1 fraction was increased after miR‐203 overexpression. (D, E) Apoptosis assay to investigate the population of apoptotic cells and viable cells of SkMCs after miR‐203 overexpression. Apoptosis rates were measured by annexin V and 7‐amino‐actinomycin D (7‐AAD) staining, and apoptotic cells were calculated as upper right and lower right (D): negative control (CTRL): right panel; miR‐203 mimic: left panel. Rate of apoptotic cells was significantly increased after miR‐203 overexpression ( P < 0.05, lower panel). The number of viable cancer cells was also calculated by MTT assay and showed that miR‐203 up‐regulation decreased the number of viable SkMCs (E). (F) Prediction of miR‐203 target gene via four different miRNA target prediction tools (miRMap, Microt4, Targetscan, and RNAhybrid). (G) Expression profile of apoptosis‐related genes about putative targets of miR‐203 showed that overexpression of miR‐203 suppressed BIRC5 expression in SkMCs. Expression profile of apoptosis‐related genes about putative targets of miR‐203 showed that overexpression of miR‐203 suppressed BIRC5 expression in SkMCs. (H) BIRC5 protein was expressed in both nucleus and cytoplasm in SkMCs and was down‐regulated in miR‐203 mimic‐transfected cells compared with negative CTRL cells. Each value represents the mean ± standard error. OD, optical density.
    Human Skeletal Muscle Cell Line Skmc, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+skmc+line/human+skeletal+muscle+myoblasts/pmc02813858-227-0-9
    Average 90 stars, based on 1 article reviews
    human skeletal muscle cell line skmc - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    skeletal muscle cell line skmc  (PromoCell)
    94
    PromoCell skeletal muscle cell line skmc
    Series of in vitro analysis using <t>human</t> <t>skeletal</t> muscle cells (SkMCs). (A) Overexpression of miR‐203 expression 48 h after transfection in <t>SkMC</t> line. (B) Effect of miR‐203 overexpression on SkMC proliferation as assessed by MTT assay. (C) Cell cycle analysis demonstrated that G 0 /G 1 fraction was increased after miR‐203 overexpression. (D, E) Apoptosis assay to investigate the population of apoptotic cells and viable cells of SkMCs after miR‐203 overexpression. Apoptosis rates were measured by annexin V and 7‐amino‐actinomycin D (7‐AAD) staining, and apoptotic cells were calculated as upper right and lower right (D): negative control (CTRL): right panel; miR‐203 mimic: left panel. Rate of apoptotic cells was significantly increased after miR‐203 overexpression ( P < 0.05, lower panel). The number of viable cancer cells was also calculated by MTT assay and showed that miR‐203 up‐regulation decreased the number of viable SkMCs (E). (F) Prediction of miR‐203 target gene via four different miRNA target prediction tools (miRMap, Microt4, Targetscan, and RNAhybrid). (G) Expression profile of apoptosis‐related genes about putative targets of miR‐203 showed that overexpression of miR‐203 suppressed BIRC5 expression in SkMCs. Expression profile of apoptosis‐related genes about putative targets of miR‐203 showed that overexpression of miR‐203 suppressed BIRC5 expression in SkMCs. (H) BIRC5 protein was expressed in both nucleus and cytoplasm in SkMCs and was down‐regulated in miR‐203 mimic‐transfected cells compared with negative CTRL cells. Each value represents the mean ± standard error. OD, optical density.
    Skeletal Muscle Cell Line Skmc, supplied by PromoCell, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+skmc+line/Human+Skeletal+Muscle+Cells/pm18606210-51-9-23
    Average 94 stars, based on 1 article reviews
    skeletal muscle cell line skmc - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    Series of in vitro analysis using human skeletal muscle cells (SkMCs). (A) Overexpression of miR‐203 expression 48 h after transfection in SkMC line. (B) Effect of miR‐203 overexpression on SkMC proliferation as assessed by MTT assay. (C) Cell cycle analysis demonstrated that G 0 /G 1 fraction was increased after miR‐203 overexpression. (D, E) Apoptosis assay to investigate the population of apoptotic cells and viable cells of SkMCs after miR‐203 overexpression. Apoptosis rates were measured by annexin V and 7‐amino‐actinomycin D (7‐AAD) staining, and apoptotic cells were calculated as upper right and lower right (D): negative control (CTRL): right panel; miR‐203 mimic: left panel. Rate of apoptotic cells was significantly increased after miR‐203 overexpression ( P < 0.05, lower panel). The number of viable cancer cells was also calculated by MTT assay and showed that miR‐203 up‐regulation decreased the number of viable SkMCs (E). (F) Prediction of miR‐203 target gene via four different miRNA target prediction tools (miRMap, Microt4, Targetscan, and RNAhybrid). (G) Expression profile of apoptosis‐related genes about putative targets of miR‐203 showed that overexpression of miR‐203 suppressed BIRC5 expression in SkMCs. Expression profile of apoptosis‐related genes about putative targets of miR‐203 showed that overexpression of miR‐203 suppressed BIRC5 expression in SkMCs. (H) BIRC5 protein was expressed in both nucleus and cytoplasm in SkMCs and was down‐regulated in miR‐203 mimic‐transfected cells compared with negative CTRL cells. Each value represents the mean ± standard error. OD, optical density.

    Journal: Journal of Cachexia, Sarcopenia and Muscle

    Article Title: Circulating miR‐203 derived from metastatic tissues promotes myopenia in colorectal cancer patients

    doi: 10.1002/jcsm.12403

    Figure Lengend Snippet: Series of in vitro analysis using human skeletal muscle cells (SkMCs). (A) Overexpression of miR‐203 expression 48 h after transfection in SkMC line. (B) Effect of miR‐203 overexpression on SkMC proliferation as assessed by MTT assay. (C) Cell cycle analysis demonstrated that G 0 /G 1 fraction was increased after miR‐203 overexpression. (D, E) Apoptosis assay to investigate the population of apoptotic cells and viable cells of SkMCs after miR‐203 overexpression. Apoptosis rates were measured by annexin V and 7‐amino‐actinomycin D (7‐AAD) staining, and apoptotic cells were calculated as upper right and lower right (D): negative control (CTRL): right panel; miR‐203 mimic: left panel. Rate of apoptotic cells was significantly increased after miR‐203 overexpression ( P < 0.05, lower panel). The number of viable cancer cells was also calculated by MTT assay and showed that miR‐203 up‐regulation decreased the number of viable SkMCs (E). (F) Prediction of miR‐203 target gene via four different miRNA target prediction tools (miRMap, Microt4, Targetscan, and RNAhybrid). (G) Expression profile of apoptosis‐related genes about putative targets of miR‐203 showed that overexpression of miR‐203 suppressed BIRC5 expression in SkMCs. Expression profile of apoptosis‐related genes about putative targets of miR‐203 showed that overexpression of miR‐203 suppressed BIRC5 expression in SkMCs. (H) BIRC5 protein was expressed in both nucleus and cytoplasm in SkMCs and was down‐regulated in miR‐203 mimic‐transfected cells compared with negative CTRL cells. Each value represents the mean ± standard error. OD, optical density.

    Article Snippet: The human SkMC line was purchased from Promo Cell (Heidelberg, Germany) and maintained in Skeletal Muscle Cell Growth Medium (Promo Cell) at 37 °C in 5% humidified CO 2 atmosphere.

    Techniques: In Vitro, Over Expression, Expressing, Transfection, MTT Assay, Cell Cycle Assay, Apoptosis Assay, Staining, Negative Control